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Embryo Culture Dish for the MIRI® TL

Parts of the Parts of the CultureCoin®

The Esco Medical CultureCoin® is an IVF culture dish designed specifically for the MIRI® TL. The MIRI® TL is Esco Medical’s time-lapse incubator that captures real-time images of developing embryos which can be viewed as time-lapse videos.The CultureCoin® is a single-use, gamma-sterilized culture dish that has oxygen plasma-treated surfaces for effective prevention of bubble formation. Its ergonomic design makes it easy for embryologists to load a total of 14 embryos while being able to safely and securely handle them inside the incubator. This culture well features a 300µm, optically clear area for embryo placement which is optimized for microscopy.

CultureCoin® Specifications

  • Overall dimensions
  • Weight empty
  • Material
  • Temperature range
  • CO2 range
  • O2 range
  • Lifetime
  • Toxicity tested
  • ⌀ 71 x 10 mm
  • 0.5 gram
  • Polystyrene
  • 25 ‐ 40 °C
  • 1.9 ‐ 10%
  • 2.0 ‐ 4.9%
  • 2 years
  • Embryo toxicity tested with thawed 1 cell mouse embryos. Expanded Blastocyst rate on the culture dish after 96 hours > 80 % (n=150)0.6 bar (8.70 PSI)

How to use the CultureCoin®

  1. Unpack the IVF culture dish inside a workstation or laminar flow hood to provide an aseptic environment.
  2. Leave the dishes inside the workstation with lids on for off-gassing for 12 hours.
  3. Pre-warm the dishes on a heated stage or inside a CO2 incubator prior to filling with pre-equilibrated media.
  4. Start filling all culture wells with approximately 25µL of pre-equilibrated IVF culture media in the workstation. The user also has an option to fill the wash wells with approximately 23µL of culture media.
  5. Immediately fill the culture area with a layer of suitable mineral or paraffin oil. Do not overfill.
  6. Check for any visible air bubbles under the microscope and remove them with a stripper tip.
  7. Put the lid on the culture dish and equilibrate for 4 hours inside a CO2 incubator.
  8. Check the culture area again for air bubbles after equilibration. Remove any bubbles with a stripper tip.
  9. Load embryos in the culture wells. Make sure to place the embryos very precisely at the center of the culture well and not on the side of the slope.
  10. Incubate the CultureCoin® containing the embryos in the MIRI® TL.